Biologie et Pathologie du Neurone

Journal articles

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Clotilde Thery ^{1, *} Kenneth Witwer ^{2, *} Elena Aikawa Maria Jose Alcaraz Johnathon Anderson Ramaroson Andriantsitohaina ³ Anna Antoniou Tanina Arab ⁴ Fabienne Archer ⁵ Georgia Atkin-Smith D Craig Ayre Jean-Marie Bach ⁶ Daniel Bachurski Hossein Baharvand Leonora Balaj Shawn Baldacchino Natalie Bauer Amy Baxter Mary Bebawy Carla Beckham Apolonija Bedina Zavec ⁷ Abderrahim Benmoussa ⁸ Anna Maria Berardi Paolo Bergese Ewa Bielska Cherie Blenkiron Sylwia Bobis-Wozowicz Eric Boilard ⁹ Wilfrid Boireau ¹⁰ Antonella Bongiovanni Francesc Borràs Steffi Bosch ¹¹ Chantal M. Boulanger ¹² Xandra Breakefield Andrew Breglio Meadhbh A Brennan ¹³ David Brigstock Alain Brisson ¹⁴ Marike Ld Broekman Jacqueline Bromberg Paulina Bryl-Górecka Shilpa Buch Amy Buck Dylan Burger Sara Busatto Dominik Buschmann Benedetta Bussolati ¹⁵ Edit Buzás James Bryan Byrd Giovanni Camussi ¹⁶ David Rf Carter Sarah Caruso Lawrence Chamley yu-Ting Chang Chihchen Chen Shuai Chen Lesley Cheng ¹⁷ Andrew Chin ¹⁸ Aled Clayton ¹⁹ Stefano Clerici Alex Cocks Emanuele Cocucci Robert Coffey Anabela Cordeiro-Da-Silva ²⁰ yvonne Couch Frank Aw Coumans Beth Coyle Rossella Crescitelli Miria Ferreira Criado Crislyn D’souza-Schorey Saumya Das Amrita Datta Chaudhuri Paola de Candia Eliezer de Santana Olivier de Wever ²¹ Hernando del Portillo Tanguy Demaret Sarah Deville Andrew Devitt Bert Dhondt ²¹ Dolores Di Vizio Lothar Dieterich Vincenza Dolo Ana Paula Dominguez Rubio Massimo Dominici ²² Mauricio Dourado Tom Ap Driedonks Filipe Duarte Heather Duncan Ramon Eichenberger Karin Ekström Samir El Andaloussi ²³ Céline Elie-Caille ¹⁰ Uta Erdbrügger Juan Falcón-Pérez ²⁴ Farah Fatima Jason Fish Miguel Flores-Bellver András Försönits Annie Frelet-Barrand ¹⁰ Fabia Fricke Gregor Fuhrmann Susanne Gabrielsson Ana Gámez-Valero Chris Gardiner Kathrin Gartner ²⁵ Raphael Gaudin yong Song Gho Bernd Giebel ²⁶ Caroline Gilbert ²⁷ Mario Gimona Ilaria Giusti Deborah Ci Goberdhan André Görgens Sharon Gorski David Greening Julia Christina Gross Alice Gualerzi Gopal Gupta Dakota Gustafson Aase Handberg Reka Haraszti Paul Harrison ²⁸ Hargita Hegyesi An Hendrix ²¹ Andrew Hill Fred Hochberg Karl F Hoffmann ²⁹ Beth Holder Harry Holthöfer ³⁰ Baharak Hosseinkhani Guoku Hu yiyao Huang Veronica Huber Stuart Hunt Ahmed Gamal-Eldin Ibrahim Tsuneya Ikezu Jameel Inal Mustafa Isin Alena Ivanova Hannah Jackson Soren Jacobsen ³¹ Steven Jay Muthuvel Jayachandran Guido Jenster Lanzhou Jiang Suzanne Johnson Jennifer Jones ³² Ambrose Jong Tijana Jovanovic-Talisman Stephanie Jung Raghu Kalluri Shin-Ichi Kano Sukhbir Kaur yumi Kawamura Evan Keller Delaram Khamari Elena Khomyakova Anastasia Khvorova Peter Kierulf Kwang Pyo Kim Thomas Kislinger Mikael Klingeborn David Klinke Miroslaw Kornek Maja Kosanović Árpád Ferenc Kovács Eva-Maria Krämer-Albers ³³ Susanne Krasemann Mirja Krause Igor Kurochkin Gina Kusuma Sören Kuypers Saara Laitinen Scott Langevin Lucia Languino Joanne Lannigan Cecilia Lässer Louise Laurent Grégory Lavieu ³⁴ Elisa Lázaro-Ibáñez Soazig Le Lay ³ Myung-Shin Lee yi Xin Fiona Lee Debora Lemos Metka Lenassi Aleksandra Leszczynska Isaac Ts Li Ke Liao Sten Libregts Erzsebet Ligeti Rebecca Lim Sai Kiang Lim Aija Linē Karen Linnemannstöns Alicia Llorente Catherine Lombard Magdalena Lorenowicz Ákos Lörincz Jan Lötvall ³⁵ Jason Lovett Michelle Lowry Xavier Loyer ¹² Quan Lu Barbara Lukomska Taral Lunavat Sybren Ln Maas Harmeet Malhi Antonio Marcilla ³⁶ Jacopo Mariani Javier Mariscal Elena Martens-Uzunova Lorena Martin-Jaular M Carmen Martinez ³ Vilma Regina Martins Mathilde Mathieu ³⁷ Suresh Mathivanan ¹⁷ Marco Maugeri Lynda Mcginnis Mark Mcvey David Meckes Katie Meehan Inge Mertens ³⁸ Valentina Minciacchi Andreas Möller Malene Møller Jørgensen Aizea Morales-Kastresana Jess Morhayim François Mullier ³⁹ Maurizio Muraca ⁴⁰ Luca Musante Veronika Mussack Dillon Muth Kathryn Myburgh Tanbir Najrana Muhammad Nawaz Irina Nazarenko Peter Nejsum ⁴¹ Christian Neri ⁴² Tommaso Neri Rienk Nieuwland Leonardo Nimrichter John Nolan ⁴³ Esther Nm Nolte-’t Hoen Nicole Noren Hooten Lorraine O’driscoll Tina O’grady Ana O’loghlen Takahiro Ochiya Martin Olivier ⁴⁴ Alberto Ortiz ⁴⁵ Luis Ortiz ⁴⁶ Xabier Osteikoetxea Ole Østergaard Matias Ostrowski Jaesung Park D. Michiel Pegtel Héctor Peinado ⁴⁷ Francesca Perut ⁴⁸ Michael Pfaffl Donald G. Phinney ⁴⁹ Bartijn Ch Pieters Ryan Pink David Pisetsky Elke Pogge von Strandmann Iva Polakovicova Ivan Kh Poon Bonita Powell Ilaria Prada Lynn Pulliam Peter Quesenberry Annalisa Radeghieri Robert Raffai Stefania Raimondo ⁵⁰ Janusz Rak ⁵¹ Marcel Ramirez Graça Raposo ⁵² Morsi Rayyan Neta Regev-Rudzki Franz Ricklefs Paul Robbins David Roberts ⁵³ Silvia Rodrigues Eva Rohde Sophie Rome ⁵⁴ Kasper Ma Rouschop Aurelia Rughetti Ashley Russell Paula Saá Susmita Sahoo ⁵⁵ Edison Salas-Huenuleo Catherine Sánchez Julie Saugstad Meike Saul Raymond Schiffelers ⁵⁶ Raphael Schneider ⁵⁷ Tine Hiorth Schøyen Aaron Scott Eriomina Shahaj Shivani Sharma Olga Shatnyeva Faezeh Shekari Ganesh Vilas Shelke Ashok Shetty Kiyotaka Shiba Pia R-M Siljander Andreia Silva Agata Skowronek Orman Snyder Rodrigo Pedro Soares ⁵⁸ Barbara Sódar Carolina Soekmadji Javier Sotillo Philip Stahl Willem Stoorvogel Shannon Stott Erwin Strasser Simon Swift Hidetoshi Tahara Muneesh Tewari Kate Timms Swasti Tiwari Rochelle Tixeira Mercedes Tkach Wei Seong Toh Richard Tomasini ⁵⁹ Ana Cláudia Torrecilhas ⁶⁰ Juan Pablo Tosar ⁶¹ Vasilis Toxavidis Lorena Urbanelli ⁶² Pieter Vader Bas Wm van Balkom Susanne van Der Grein ⁶³ Jan van Deun ²¹ Martijn Jc van Herwijnen Kendall van Keuren-Jensen Guillaume van Niel ⁶⁴ Martin van Royen Andre van Wijnen M Helena Vasconcelos Ivan Vechetti Tiago Veit Laura Vella Emilie Velot ⁶⁵ Frederik Verweij ⁶⁴ Beate Vestad Jose Viñas Tamás Visnovitz Krisztina Vukman Jessica Wahlgren Dionysios Watson Marca Hm Wauben Alissa Weaver Jason Webber Viktoria Weber Ann M. Wehman ⁶⁶ Daniel Weiss ⁶⁷ Joshua Welsh Sebastian Wendt Asa Wheelock Zoltán Wiener Leonie Witte Joy Wolfram Angeliki Xagorari Patricia Xander Jing Xu ⁶⁸ Xiaomei yan ⁶⁹ María yáñez-Mó Hang yin ⁷⁰ yuana yuana Valentina Zappulli Jana Zarubova Vytautas Žėkas Jian-ye Zhang Zezhou Zhao Lei Zheng Alexander Zheutlin Antje Zickler ⁷¹ Pascale Zimmermann ⁵⁹ Angela Zivkovic Davide Zocco Ewa Zuba-Surma

* Corresponding author

1 Immunité et cancer

2 Johns Hopkins University School of Medicine [Baltimore]

3 SOPAM - Stress Oxydant et Pathologies Métaboliques

4 PRISM - Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192

5 IVPC - Infections Virales et Pathologie Comparée - UMR 754

6 IECM - Immuno-Endocrinologie Cellulaire et Moléculaire [Nantes]

7 Department for Molecular Biology and Nanobiotechnology

8 BIOTARGEN - Biologie, génétique et thérapies ostéoarticulaires et respiratoires

9 IPMC - Institut de pharmacologie moléculaire et cellulaire

10 FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174)

11 IECM - Immuno-Endocrinologie Cellulaire et Moléculaire

12 PARCC - UMR-S U970 - Paris-Centre de Recherche Cardiovasculaire

13 PhAN - Physiopathologie des Adaptations Nutritionnelles

14 IECB - Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie

15 Molecular Biotechnology Center

16 UNITO - Università degli studi di Torino = University of Turin

17 Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science

18 Centre for Sustainable Tropical Fisheries and Aquaculture

19 Department of Oncology - Pathology

20 Departamento de Ciências Biológicas

21 Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Cancer Research Institute Ghent (CRIG)

22 Laboratory of Cellular Therapy - Department of Medical and Surgical Sciences for Children and Adults [Modena, Italy]

23 Clinical Research Center, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden

24 Center for Cooperative Research in Biosciences (CIC bioGUNE)

25 Partner site Munich

26 Institute for Transfusion Medicine, University Hospital Essen

27 MECADEV - Mécanismes Adaptatifs et Evolution

28 Psychiatry

29 IBERS - Institute of Biological, Environmental and Rural Sciences

30 Department of Bacteriology and Immunology [Helsinki]

31 Rigshospitalet [Copenhagen]

32 Dalhousie University [Halifax]

33 Department of Biology, Molecular Cell Biology, University of Mainz

34 Glycobiologie et signalisation cellulaire

35 Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition

36 Universidad de Alicante

37 UPEC ESPE Créteil - École supérieure du professorat et de l'éducation - Académie de Créteil

38 UA - University of Antwerp

39 UCL - Université Catholique de Louvain = Catholic University of Louvain

40 Research Institute

41 Department of Veterinary Disease Biology [Copenhagen]

42 Brain-C - Biologie et Pathologie du Neurone

B2A - Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing

43 Department of Mathematics and Statistics

44 University of Pretoria [South Africa]

45 EIVP - Ecole des Ingénieurs de la Ville de Paris

46 UPF - Universitat Pompeu Fabra [Barcelona]

47 Instituto de Investigaciones Biomedicas

48 Istituto Ortopedico Rizzoli

49 Department of Molecular Therapeutics

50 LIG - Laboratoire d'Informatique de Grenoble

51 Montreal Children's Hospital

52 CDC - Compartimentation et dynamique cellulaires

53 NCI-NIH - National Cancer Institute [Bethesda]

54 CarMeN - Cardiovasculaire, métabolisme, diabétologie et nutrition

55 Cardiovascular Research Center

56 University Medical Center [Utrecht]

57 University of Toronto

58 Fiocruz Minas - René Rachou Research Center / Instituto René Rachou [Belo Horizonte, Brésil]

59 CRCM - Centre de Recherche en Cancérologie de Marseille

60 Unifesp - Federal University of Sao Paulo

61 Functional Genomics / Genómica Funcional [Montevideo]

62 Dipartimento di Medicina Sperimentale e Scienze Biochimiche

63 Hospital Santa Cristina Instituto de Investigación Sanitaria Princesa C

64 CDC - Compartimentation et dynamique cellulaires

65 IMoPA - Ingénierie Moléculaire et Physiopathologie Articulaire

66 Department of Physiology

67 University of Vermont [Burlington]

68 Peking University [Beijing]

69 SDAU - Shandong Agricultural University

70 Institute of Chinese Medical Sciences - State Key Laboratory of Quality Research in Chinese Medicine Taipa, Macau SAR,

71 Human Genetics

Abstract : The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Keywords : ectosomes exosomes extracellular vesicles guidelines microparticles microvesicles minimal information requirements reproducibility rigor standardization

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Life Sciences [q-bio] / Cellular Biology

Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02323217
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Submitted on : Wednesday, March 10, 2021 - 3:17:09 PM
Last modification on : Saturday, June 25, 2022 - 9:15:31 PM
Long-term archiving on: : Friday, June 11, 2021 - 6:59:46 PM