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Distribution of Achromobacter species in 12 French cystic fibrosis centers in 2020 by a retrospective MALDI-TOF MS spectrum analysis

Thomas Garrigos 1, 2 Manon Dollat 1 Arnaud Magallon 1, 2 Anaïs Folletet 1 Julien Bador 2, 1 Maryam Abid 3, 4 Marlène Amara 5 Clémence Beauruelle 6 Olivier Belmonte 7 Pierre Boyer 8 Emilie Cardot-Martin 9 Anne-Gaëlle Cauchie 10 Sylvie Colin de Verdière 11 Claire Daurel 12 Cécile Gaudru 13 Farida Hamdad 14 Geneviève Héry-Arnaud 15 Baptiste Hoellinger 16 Claudie Lamoureux 6, 17 Marie-Frédérique Lartigue 18, 3, 4 Damasie Malandain 19 Océane Marchand 20 Caroline Piau 21 Sandrine Picot 22 Hélène Revillet 23, 24, 13 Zeina Sabouni 20 Catherine Neuwirth 1, 2 Lucie Amoureux 1, 2, * 
Résumé : Achromobacter spp. are nonfermenting Gram-negative bacilli mainly studied among cystic fibrosis (CF) patients. The identification of the 19 species within the genus is time-consuming (nrdA-sequencing), thus data concerning the distribution of the species are limited to specific studies. Recently, we built a database using MALDI-TOF mass spectrometry (MS) (Bruker) that allows rapid and accurate species identification and detection of the multiresistant epidemic clones: A xylosoxidans ST137 spreading among CF patients in various French and Belgium centers, and A. ruhlandii DES in Denmark. Here, we first assessed whether species identification could be achieved with our database solely by analysis of MS spectra without availability of isolates. Then, we conducted a multicentric study describing the distribution of Achromobacter species and of the clone ST137 among French CF centers. We collected and analyzed with our local database the spectra of Achromobacter isolates from 193 patients (528 samples) from 12 centers during 2020. In total, our approach enabled to conclude for 502/528 samples (95.1%), corresponding to 181 patients. Eleven species were detected, only five being involved in chronic colonization, A. xylosoxidans (86.4%), A. insuavis (9.1%), A. mucicolens (2.3%), A. marplatensis (1.1%) and A. genogroup 3 (1.1%). This study confirmed the high prevalence of A. xylosoxidans in chronic colonizations and the circulation of the clone A. xylosoxidans ST137 in France: four patients in two centers. The present study is the first to report the distribution of Achromobacter species from CF patients samples using retrospective MALDI-TOF/MS data. This easy approach could enable future large-scale epidemiological studies.
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Submitted on : Monday, June 13, 2022 - 10:08:07 AM
Last modification on : Thursday, August 4, 2022 - 5:27:53 PM

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Thomas Garrigos, Manon Dollat, Arnaud Magallon, Anaïs Folletet, Julien Bador, et al.. Distribution of Achromobacter species in 12 French cystic fibrosis centers in 2020 by a retrospective MALDI-TOF MS spectrum analysis. 2022, ⟨10.1128/jcm.02422-21⟩. ⟨hal-03685833⟩

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