Skip to Main content Skip to Navigation
Journal articles

Transcriptional Changes Involved in Atrophying Muscles during Prolonged Fasting in Rats

Abstract : Abstract: Food deprivation resulting in muscle atrophy may be detrimental to health. To better understand how muscle mass is regulated during such a nutritional challenge, the current study deciphered muscle responses during phase 2 (P2, protein sparing) and phase 3 (P3, protein mobilization) of prolonged fasting in rats. This was done using transcriptomics analysis and a series of biochemistry measurements. The main findings highlight changes for plasma catabolic and anabolic stimuli, as well as for muscle transcriptome, energy metabolism, and oxidative stress. Changes were generally consistent with the intense use of lipids as fuels during P2. They also reflected increased muscle protein degradation and repressed synthesis, in a more marked manner during P3 than P2 compared to the fed state. Nevertheless, several unexpected changes appeared to be in favor of muscle protein synthesis during fasting, notably at the level of the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway, transcription and translation processes, and the response to oxidative stress. Such mechanisms might promote protein sparing during P2 and prepare the restoration of the protein compartment during P3 in anticipation of food intake for optimizing the e ects of an upcoming refeeding, thereby promoting body maintenance and survival. Future studies should examine relevance of such targets for improving nitrogen balance during catabolic diseases.
Document type :
Journal articles
Complete list of metadatas

Cited literature [117 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02962218
Contributor : Brigitte Gaillard <>
Submitted on : Friday, October 9, 2020 - 9:23:52 AM
Last modification on : Thursday, November 19, 2020 - 9:34:03 AM

File

ijms-21-05984.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Marianne Ibrahim, Thierry Wasselin, Etienne Challet, Alain van Dorsselaer, Yvon Le Maho, et al.. Transcriptional Changes Involved in Atrophying Muscles during Prolonged Fasting in Rats. International Journal of Molecular Sciences, MDPI, 2020, 21 (17), pp.5984. ⟨10.3390/ijms21175984⟩. ⟨hal-02962218⟩

Share

Metrics

Record views

39

Files downloads

44