Introducing a targeted mutation in the Cyclin G gene of Drosophila melanogaster by developing a specific zinc finger nuclease

Delphine Dardalhon-Cumenal (1) , D Cuménal-Dardalhon , F Faradji , V Ribeiro , A Coléno-Costes , S Bloyer , E Mouchel-Vielh , N Randsholt , F Peronnet

1 LBD - Laboratoire de Biologie du Développement [IBPS]

We thank Pr. D. Carroll for Strains and Reagents. Understanding the role of Drosophila melanogaster Cyclin G Different results show the involvement of Cyclin G in:-Transcriptional regulation (Salvaing et al., 2008)-Growth control and cell proliferation (F. Faradji et al., in preparation). • We have got different strains allowing Cyclin G (CycG) overexpression (S. Bloyer, unpublished), or inactivation by RNAi (Salvaing et al., 2008) but no mutant has been obtained till now. I-INTRODUCTION II-Materials and Methods B-Non-Homologous End-Joining (NHEJ)

Domaines: Sciences du Vivant [q-bio]

Delphine Dardalhon-Cumenal, D Cuménal-Dardalhon, F Faradji, V Ribeiro, A Coléno-Costes, et al.. Introducing a targeted mutation in the Cyclin G gene of Drosophila melanogaster by developing a specific zinc finger nuclease. Modification contrôlée du génome à l'aide d'endonucléases à façon, 2010, Saint Raphaël, France. ⟨hal-03564679⟩