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A supernumerary “B-sex” chromosome drives male sex determination in the Pachón cavefish, Astyanax mexicanus

Boudjema Imarazene 1, 2 Kang Du 3 Séverine Beille 1 Elodie Jouanno 1 Romain Feron 1, 4, 5 Qiaowei Pan 1, 4 Jorge Torres-Paz 2 Céline Lopez-Roques 6 Adrien Castinel 6 Lisa Gil 6 Claire Kuchly 6 Cécile Donnadieu 6 Hugues Parrinello 7 Laurent Journot 7 Cédric Cabau 8, 9 Margot Zahm 10, 9 Christophe Klopp 10, 9 Tomáš Pavlica 11, 12 Ahmed Al-Rikabi 13 Thomas Liehr 13 Sergey Simanovsky 14 Joerg Bohlen 11 Alexandr Sember 11 Julie Perez 15 Frédéric Veyrunes 15 Thomas Mueller 16 John Postlethwait 17 Manfred Schartl 3, 16 Amaury Herpin 1 Sylvie Rétaux 2 Yann Guiguen 1
Abstract : Sex chromosomes are generally derived from a pair of classical type-A chromosomes, and relatively few alternative models have been proposed up to now. B chromosomes (Bs) are supernumerary and dispensable chromosomes with non-Mendelian inheritance found in many plant and animal species that have often been considered as selfish genetic elements that behave as genome parasites. The observation that in some species Bs can be either restricted or predominant in one sex raised the interesting hypothesis that Bs could play a role in sex determination. The characterization of putative B master sex-determining (MSD) genes, however, has not yet been provided to support this hypothesis. Here, in Astyanax mexicanus cavefish originating from Pachón cave, we show that Bs are strongly male predominant. Based on a high-quality genome assembly of a B-carrying male, we characterized the Pachón cavefish B sequence and found that it contains two duplicated loci of the putative MSD gene growth differentiation factor 6b (gdf6b). Supporting its role as an MSD gene, we found that the Pachón cavefish gdf6b gene is expressed specifically in differentiating male gonads, and that its knockout induces male-to-female sex reversal in B-carrying males. This demonstrates that gdf6b is necessary for triggering male sex determination in Pachón cavefish. Altogether these results bring multiple and independent lines of evidence supporting the conclusion that the Pachón cavefish B is a “B-sex” chromosome that contains duplicated copies of the gdf6b gene, which can promote male sex determination in this species.
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https://hal.inrae.fr/hal-03337251
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Submitted on : Tuesday, September 14, 2021 - 11:06:19 AM
Last modification on : Wednesday, November 17, 2021 - 12:31:41 PM

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Boudjema Imarazene, Kang Du, Séverine Beille, Elodie Jouanno, Romain Feron, et al.. A supernumerary “B-sex” chromosome drives male sex determination in the Pachón cavefish, Astyanax mexicanus. Current Biology - CB, Elsevier, 2021, ⟨10.1016/j.cub.2021.08.030⟩. ⟨hal-03337251⟩

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