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Mechanism and dynamics of fatty acid photodecarboxylase

Damien Sorigué 1 Kyprianos Hadjidemetriou 2 S. Blangy 1 G. Gotthard 3 A. Bonvalet 4 Nicolas Coquelle 5, 2 P. Samire 1, 6 Alexey Aleksandrov 4 L. Antonucci 4 A. Benachir 4 S. Boutet 7 Martin Byrdin 2 Marco Cammarata 8 S. Carbajo 7 S. Cuiné 1 R. Doak 9 L. Foucar 9 A. Gorel 9 M. Grünbein 9 E. Hartmann 9 R. Hienerwadel 10, 11 M. Hilpert 9 M. Kloos 9 T. Lane 7 B. Légeret 1 P. Legrand 12 Y. Li-Beisson 1 S. Moulin 1 D. Nurizzo 3 G. Peltier 1 Giorgio Schirò 2 R. Shoeman 9 M. Sliwa 13 X. Solinas 4 B. Zhuang 4, 6 T. Barends 9 Jacques-Philippe Colletier 2 M. Joffre 4 Antoine Royant 2, 3 C. Berthomieu 10, 14, * Martin Weik 2, * T. Domratcheva 9, 15, * K. Brettel 6 Marten H Vos 4, * I. Schlichting 9, * P. Arnoux 10, 16, * P. Müller 6, * F. Beisson 1, *
Abstract : Fatty acid photodecarboxylase (FAP) is a photoenzyme with potential green chemistry applications. By combining static, time-resolved, and cryotrapping spectroscopy and crystallography as well as computation, we characterized Chlorella variabilis FAP reaction intermediates on time scales from subpicoseconds to milliseconds. High-resolution crystal structures from synchrotron and free electron laser x-ray sources highlighted an unusual bent shape of the oxidized flavin chromophore. We demonstrate that decarboxylation occurs directly upon reduction of the excited flavin by the fatty acid substrate. Along with flavin reoxidation by the alkyl radical intermediate, a major fraction of the cleaved carbon dioxide unexpectedly transformed in 100 nanoseconds, most likely into bicarbonate. This reaction is orders of magnitude faster than in solution. Two strictly conserved residues, R451 and C432, are essential for substrate stabilization and functional charge transfer.
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Damien Sorigué, Kyprianos Hadjidemetriou, S. Blangy, G. Gotthard, A. Bonvalet, et al.. Mechanism and dynamics of fatty acid photodecarboxylase. Science, American Association for the Advancement of Science, 2021, 372 (6538), pp.eabd5687. ⟨10.1126/science.abd5687⟩. ⟨hal-03208662⟩

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