Skip to Main content Skip to Navigation
Journal articles

Dynamics of Tpm1.8 domains on actin filaments with single-molecule resolution

Abstract : Tropomyosins regulate dynamics and functions of the actin cytoskeleton by forming long chains along the two strands of actin filaments that act as gatekeepers for the binding of other actin-binding proteins. The fundamental molecular interactions underlying the binding of tropomyosin to actin are still poorly understood. Using microfluidics and fluorescence microscopy, we observed the binding of fluorescently labelled tropomyosin isoform Tpm1.8 to unlabelled actin filaments in real time. This approach in conjunction with mathematical modeling enabled us to quantify the nucleation, assembly and disassembly kinetics of Tpm1.8 on single filaments and at the single molecule level. Our analysis suggests that Tpm1.8 decorates the two strands of the actin filament independently. Nucleation of a growing tropomyosin domain proceeds with high probability as soon as the first Tpm1.8 molecule is stabilised by the addition of a second molecule, ultimately leading to full decoration of the actin filament. In addition, Tpm1.8 domains are asymmetrical, with enhanced dynamics at the edge oriented towards the barbed end of the actin filament. The complete description of Tpm1.8 kinetics on actin filaments presented here provides molecular insight into actin-tropomyosin filament formation and the role of tropomyosins in regulating actin filament dynamics. .
Document type :
Journal articles
Complete list of metadatas
Contributor : Guillaume Romet-Lemonne <>
Submitted on : Monday, December 28, 2020 - 12:06:27 PM
Last modification on : Thursday, January 7, 2021 - 3:38:14 AM


Files produced by the author(s)




Ilina Bareja, Hugo Wioland, Miro Janco, Philip Nicovich, Antoine Jégou, et al.. Dynamics of Tpm1.8 domains on actin filaments with single-molecule resolution. Molecular Biology of the Cell, American Society for Cell Biology, 2020, 31 (22), pp.2452-2462. ⟨10.1091/mbc.E19-10-0586⟩. ⟨hal-03087728⟩



Record views


Files downloads