Skip to Main content Skip to Navigation
Journal articles

Cholesterol-pyrene as a probe for cholesterol distribution on ordered and disordered membranes: Determination of spectral wavelengths

Abstract : Biological membranes contain a large variety of lipids species compartmentalized in different domains heterogeneous in size, composition and dynamics. Cholesterol induces membrane ordered domains thanks to its affinity for saturated lipids. Membrane domains had been studied with fluorescent probes either linked to phospholipids and proteins or as individual fluorophore. However, no efficient formulation of a cholesterol probe has been available so far. Herein, we described a cholesterol-pyrene probe behaviour in heterogeneous membranes. We characterised the pyrene fluorescence spectra in liquid-ordered (Lo) and liquid-disordered (Ld) membranes. Using statistical multivariate analysis, we found out the most appropriate wavelengths for membrane domains studies. 373 nm and 379 nm were the most discriminant wavelengths to follow the liquid-ordered and the liquid-disordered environments. Cholesterol clustering behaviour was quantified by the modulation of the cholesterol -pyrene excimers peak (474 nm). In liquid-ordered membranes at low temperature, cholesterol-pyrene was found as multimers and as monomers. At high temperature, the liquid ordered status of the membrane decreases and cholesterol-pyrene tends to cluster. In liquid-disordered membranes, cholesterol-pyrene was present mostly as monomers and the small quantity of excimers increased with temperature. Cholesterol-pyrene was used to test the ceramide effect on membranes, and presented a behaviour in agreement with the cholesterol behaviour reported in the literature. Overall, the presented data show that cholesterol -pyrene is an efficient sensor to study liquid ordered and liquid disordered organisation in membranes.
Complete list of metadata

Cited literature [51 references]  Display  Hide  Download
Contributor : Jesus Ayala-Sanmartin Connect in order to contact the contributor
Submitted on : Monday, October 28, 2019 - 12:26:18 PM
Last modification on : Wednesday, November 17, 2021 - 12:33:18 PM
Long-term archiving on: : Wednesday, January 29, 2020 - 2:31:22 PM


2018 PyMetChol.pdf
Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License



Claudia Almeida, Anaë Lle de Wreede, Antonin Lamazière, Jesus Ayala-Sanmartin. Cholesterol-pyrene as a probe for cholesterol distribution on ordered and disordered membranes: Determination of spectral wavelengths. PLoS ONE, Public Library of Science, 2018, ⟨10.1371/journal.pone.0201373⟩. ⟨hal-02335484⟩



Record views


Files downloads