Improved Molecular Detection of Dengue Virus Serotype 1 Variants

Jean-Marc Reynes (1) , Sivuth Ong (1) , Channa Mey (1) , Chantha Ngan (2) , Stefan Hoyer (3) , Amadou Alpha Sall (1)

1 Institut Pasteur du Cambodge
2 Ministry of Health [Phnom Penh]
3 WHO - World Health Organization [Phnom Penh]

The dengue virus molecular typing method described by Lanciotti and coworkers (R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A.Vorndam, J. Clin. Microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. However, it failed to detect DENV-1 variants in Cambodia due to a point mutation. We describe an improvement of the method that allows the detection of additional DENV-1 strains, including potential variants.

Domaines: Maladies infectieuses; Virologie; Santé publique et épidémiologie

Jean-Marc Reynes, Sivuth Ong, Channa Mey, Chantha Ngan, Stefan Hoyer, et al.. Improved Molecular Detection of Dengue Virus Serotype 1 Variants. Journal of Clinical Microbiology, 2003, 41 (8), pp.3864 - 3867. ⟨10.1128/JCM.41.8.3864-3867.2003⟩. ⟨pasteur-01836736⟩