De Novo Transcriptomic Approach to Study Thyroid Hormone Receptor Action in Non-mammalian Models

Nicolas Buisine (1, 2) , Gwenneg Kerdivel , Laurent Sachs (1, 2)

1 ERE - Evolution des régulations endocriniennes
2 PhyMA - Physiologie moléculaire et adaptation

Thyroid hormones are pleiotropic hormones involved in chordates physiology. Understanding their functions and mechanisms is also instrumental to diagnose dys-regulations and get a predictive power that can applied to medicine, ecology, etc. Today, high-throughput sequencing technologies offer the opportunity to address this issue not only in model organisms but also in non-model organisms. Here, we describe a method that makes use of RNA-seq to address differential expression analysis in non-model organism.

Mots clés: Transcriptome annotation; Thyroid hormones; Transcriptome assembly; transcriptome assembly; Differential expression analysis; transcriptome annotation; differential expression analysis

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Nicolas Buisine, Gwenneg Kerdivel, Laurent Sachs. De Novo Transcriptomic Approach to Study Thyroid Hormone Receptor Action in Non-mammalian Models. Thyroid Hormone Nuclear Receptor. Methods and Protocols, 1801, 2018, ⟨10.1007/978-1-4939-7902-8_21⟩. ⟨hal-02864269⟩