A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle

Glenda Comai 1 Eglantine Heude 2, 1 Sebastian Mella 1 Sylvain Paisant 1 Francesca Pala 1, 3 Mirialys Gallardo 4 Francina Langa 5 Gabrielle Kardon 4 Swetha Gopalakrishnan 1, 6 Shahragim Tajbakhsh 1, *
* Corresponding author
1 Cellules Souches et Développement
2 AdV - Département Adaptations du vivant
3 LCIM - Laboratory of Clinical Immunology and Microbiology [Bethesda, MA, USA]
4 Department of Human Genetics [Salt Lake City, UT, USA]
5 Plate-Forme Technologique Centre d’Ingénierie Génétique Murine [Institut Pasteur, Paris]
6 HiLIFE - Helsinki Institute of Life Science [Helsinki, Finlande]
Abstract : In most vertebrates, the upper digestive tract is composed of muscularized jaws linked to the esophagus that permits food ingestion and swallowing. Masticatory and esophagus striated muscles (ESM) share a common cardiopharyngeal mesoderm (CPM) origin, however ESM are unusual among striated muscles as they are established in the absence of a primary skeletal muscle scaffold. Using mouse chimeras, we show that the transcription factors Tbx1 and Isl1 are required cell-autonomously for myogenic specification of ESM progenitors. Further, genetic loss-of-function and pharmacological studies point to MET/HGF signaling for antero-posterior migration of esophagus muscle progenitors, where Hgf ligand is expressed in adjacent smooth muscle cells. These observations highlight the functional relevance of a smooth and striated muscle progenitor dialogue for ESM patterning. Our findings establish a Tbx1-Isl1-Met genetic hierarchy that uniquely regulates esophagus myogenesis and identify distinct genetic signatures that can be used as framework to interpret pathologies arising within CPM derivatives.
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Journal articles
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https://hal-mnhn.archives-ouvertes.fr/mnhn-02295466
Contributor : Eglantine Heude <>
Submitted on : Tuesday, September 24, 2019 - 11:21:31 AM
Last modification on : Thursday, September 26, 2019 - 3:34:09 PM

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Glenda Comai, Eglantine Heude, Sebastian Mella, Sylvain Paisant, Francesca Pala, et al.. A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle. eLife, eLife Sciences Publication, 2019, 8, pp.e47460. ⟨10.7554/eLife.47460⟩. ⟨mnhn-02295466⟩

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