High BrdU Sensitivity of Passeriformes Chromosomes: Conservation of BrdU-Sensitive Fragile Sites on Their Z Chromosomes during Evolution - MNHN - Muséum national d'Histoire naturelle Access content directly
Journal Articles Cytogenetic and Genome Research Year : 2019

High BrdU Sensitivity of Passeriformes Chromosomes: Conservation of BrdU-Sensitive Fragile Sites on Their Z Chromosomes during Evolution

Abstract

Amongst 15 species of birds, belonging to 7 orders, recurrent breakages evocating the presence of fragile sites were detected in the chromosomes of the 5 species belonging to Passeriformes. These breaks appear when BrdU (5-bromodeoxyuridine) is added to the cell culture medium, at a dose inefficient for inducing chromosome structure alterations in other birds and mammals. They involve, similarly in male and female, 3 loci on the Z chromosome of 3 species of the genus Turdus (Turdidae). The labeling by BrdU antibody confirms the correlation between BrdU incorporation into DNA and breakage, especially around and in the sites of breakage. Thus, 3 BrdU-sensitive fragile sites are present on the Z chromosome of these birds. Three fragile sites were also detected at different locations in the Z chromosomes of the European Robin (Erithacus rubecula Muscicapidae), which suggests that a structural rearrangement occurred during the evolution of Turdidae and Muscicapidae. Chromosome banding confirms this interpretation. Finally, in the more distantly related species Parus major (Paridae), the almost acrocentric Z chromosome displays a single BrdU-sensitive fragile site on its short arm and the W appears to be pulverized by BrdU incorporation. Although it cannot be excluded that the BrdU-sensitive fragile sites may be involved in rearrangements, their conservation in many species and possibly all Passeriformes, provides evidence that they do not constitute a pejorative character during evolution.
Fichier principal
Vignette du fichier
High BrdU-sensitivity....pdf (869.17 Ko) Télécharger le fichier
Figure 1ABCD.jpg (450.34 Ko) Télécharger le fichier
Figure 2ABCD.jpg (246.84 Ko) Télécharger le fichier
Figure 3ABCD (1).jpg (1.17 Mo) Télécharger le fichier
Figure 4AB (1).jpg (536.97 Ko) Télécharger le fichier
Figure 4AB (2).jpg (536.97 Ko) Télécharger le fichier
Figure 5.jpg (899.99 Ko) Télécharger le fichier
Table 1 ok.pdf (217.64 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

mnhn-02365422 , version 1 (15-11-2019)

Identifiers

Cite

Michèle Gerbault-Seureau, Jérôme Fuchs, Bernard Dutrillaux. High BrdU Sensitivity of Passeriformes Chromosomes: Conservation of BrdU-Sensitive Fragile Sites on Their Z Chromosomes during Evolution. Cytogenetic and Genome Research, 2019, ⟨10.1159/000499590⟩. ⟨mnhn-02365422⟩
59 View
161 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More