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Organisation of the microtubular cytoskeleton in protoplasts from Palmaria palmata (Palmariales, Rhodophyta)

Abstract : Confocal laser scanning microscopy was used to investigate the three-dimensional organisation of the microtubular cytoskeleton of protoplasts isolated from the cortex and medulla of the red alga Palmaria palmata. Immunofluorescence labelling revealed the presence of abundant microtubules in interphase cells. Cortexderived and medulla-derived protoplasts each exhibited a distinctive arrangement of microtubules. In cortexderived protoplasts, a continuous network of cytoplasmic microtubules connected the peripheral array to the array which surrounded the single nucleus at the centre of the cell. At telophase, the microtubular cytoskeleton was reorganised into a network apparently involved in cytokinesis. In the multi-nucleate medulla-derived protoplasts, each nucleus, located near the plasma membrane, was surrounded by microtubules. A uniformly arranged cortical array of microtubules was observed in both cortex-derived and medulla-derived protoplasts during interphase and we hypothesise that it likely plays a role in the biogenesis of the cell wall in red algal cells.
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Submitted on : Wednesday, March 4, 2020 - 3:27:19 PM
Last modification on : Friday, September 18, 2020 - 2:35:00 PM

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Line Le Gall, Anne-Marie Rusig, Joël Cosson. Organisation of the microtubular cytoskeleton in protoplasts from Palmaria palmata (Palmariales, Rhodophyta). Botanica Marina, De Gruyter, 2004, 47 (3), ⟨10.1515/BOT.2004.024⟩. ⟨mnhn-02498568⟩

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