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Rad50 Is Not Essential for the Mre11-Dependent Repair of DNA Double-Strand Breaks in Halobacterium sp. Strain NRC-1

Abstract : The genome of the halophilic archaeon Halobacterium sp. strain NRC-1 encodes homologs of the eukaryotic Mre11 and Rad50 proteins, which are involved in the recognition and end processing of DNA double-strand breaks in the homologous recombination repair pathway. We have analyzed the phenotype of Halobacterium deletion mutants lacking mre11 and/or rad50 after exposure to UV-C radiation, an alkylating agent (N-methyl-N-nitro-N-nitrosoguanidine), and ␥ radiation, none of which resulted in a decrease in survival of the mutant strains compared to that of the background strain. However, a decreased rate of repair of DNA double-strand breaks in strains lacking the mre11 gene was observed using pulsed-field gel electrophoresis. These observations led to the hypothesis that Mre11 is essential for the repair of DNA double-strand breaks in Halobacterium, whereas Rad50 is dispensable. This is the first identification of a Rad50-independent function for the Mre11 protein, and it represents a shift in the Archaea away from the eukaryotic model of homologous recombination repair of DNA double-strand breaks.
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https://hal-mnhn.archives-ouvertes.fr/mnhn-02862428
Contributor : Adrienne Kish <>
Submitted on : Tuesday, June 9, 2020 - 3:07:35 PM
Last modification on : Tuesday, June 9, 2020 - 3:07:36 PM

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Adrienne Kish, J. Diruggiero. Rad50 Is Not Essential for the Mre11-Dependent Repair of DNA Double-Strand Breaks in Halobacterium sp. Strain NRC-1. Journal of Bacteriology, American Society for Microbiology, 2008, 190 (15), pp.5210-5216. ⟨10.1128/JB.00292-08⟩. ⟨mnhn-02862428⟩

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