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Functional kleptoplasts intermediate incorporation of carbon and nitrogen in cells of the Sacoglossa sea slug Elysia viridis

Abstract : Article Open Access Published: 29 June 2020 Functional kleptoplasts intermediate incorporation of carbon and nitrogen in cells of the Sacoglossa sea slug Elysia viridis Sónia Cruz, Charlotte LeKieffre, Paulo Cartaxana, Cédric Hubas, Najet Thiney, Sofie Jakobsen, Stéphane Escrig, Bruno Jesus, Michael Kühl, Ricardo Calado & Anders Meibom Scientific Reports volume 10, Article number: 10548 (2020) Cite this article 69 Accesses 5 Altmetric Metricsdetails Abstract Some sacoglossan sea slugs incorporate intracellular functional algal chloroplasts, a process termed kleptoplasty. “Stolen” chloroplasts (kleptoplasts) can remain photosynthetically active up to several months, contributing to animal nutrition. Whether this contribution occurs by means of translocation of photosynthesis-derived metabolites from functional kleptoplasts to the animal host or by simple digestion of such organelles remains controversial. Imaging of 13C and 15N assimilation over a 12-h incubation period of Elysia viridis sea slugs showed a light-dependent incorporation of carbon and nitrogen, observed first in digestive tubules and followed by a rapid accumulation into chloroplast-free organs. Furthermore, this work revealed the presence of 13C-labeled long-chain fatty acids (FA) typical of marine invertebrates, such as arachidonic (20:4n-6) and adrenic (22:4n-6) acids. The time frame and level of 13C- and 15N-labeling in chloroplast-free organs indicate that photosynthesis-derived primary metabolites were made available to the host through functional kleptoplasts. The presence of specific 13C-labeled long-chain FA, absent from E. viridis algal food, indicates animal based-elongation using kleptoplast-derived FA precursors. Finally, carbon and nitrogen were incorporated in organs and tissues involved in reproductive functions (albumin gland and gonadal follicles), implying a putative role of kleptoplast photosynthesis in the reproductive fitness of the animal host.
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https://hal-mnhn.archives-ouvertes.fr/mnhn-02888478
Contributor : Cédric Hubas <>
Submitted on : Friday, July 3, 2020 - 9:31:05 AM
Last modification on : Sunday, October 25, 2020 - 7:06:53 AM

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Sónia Cruz, Charlotte Lekieffre, Paulo Cartaxana, Cédric Hubas, Najet Thiney, et al.. Functional kleptoplasts intermediate incorporation of carbon and nitrogen in cells of the Sacoglossa sea slug Elysia viridis. Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-020-66909-7⟩. ⟨mnhn-02888478⟩

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