Skip to Main content Skip to Navigation
Journal articles

Demystifying biotrophs: FISHing for mRNAs to decipher plant and algal pathogen–host interaction at the single cell level

Abstract : plant-pathogen interactions follow spatial and temporal developmental dynamics where gene expression in pathogen and host undergo crucial changes. therefore, it is of great interest to detect, quantify and localise where and when key genes are active to understand these processes. Many pathosystems are not accessible for genetic amendments or other spatially-resolved gene expression monitoring methods. Here, we adapt single molecule fiSH techniques to demonstrate the presence and activity of mRnAs at the single-cell level using phytomyxids in their plant and algal host in lab and field material. This allowed us to monitor and quantify the expression of genes from the clubroot pathogen Plasmodiophora brassicae, several species of its Brassica hosts, and of several brown algae, including the genome model Ectocarpus siliculosus, infected with the phytomyxid Maullinia ectocarpii. We show that mRnAs are localised along a spatiotemporal gradient, thus providing a proof-of-concept of the usefulness of single-molecule fiSH to increase knowledge about the interactions between plants, algae and phytomyxids. the methods used are easily applicable to any interaction between microbes and their algal or plant host, and have therefore the potential to rapidly increase our understanding of key, spatially-and temporally-resolved processes underpinning complex plant-microbe interactions.
Document type :
Journal articles
Complete list of metadatas

Cited literature [60 references]  Display  Hide  Download

https://hal-mnhn.archives-ouvertes.fr/mnhn-02931536
Contributor : Claire Gachon <>
Submitted on : Monday, September 7, 2020 - 9:06:51 AM
Last modification on : Wednesday, September 9, 2020 - 10:29:49 AM

File

2020 SciRep Julia Sigrid FISHi...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Julia Badstöber, Claire Gachon, Jutta Ludwig-Müller, Adolf Sandbichler, Sigrid Neuhauser. Demystifying biotrophs: FISHing for mRNAs to decipher plant and algal pathogen–host interaction at the single cell level. Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-020-70884-4⟩. ⟨mnhn-02931536⟩

Share

Metrics

Record views

10

Files downloads

13