Somatostatin 1.1 contributes to the innate exploration of zebrafish larva

Feng B Quan (1, 2) , Laura Desban (1) , Olivier Mirat (1) , Maxime Kermarquer (1) , Julian Roussel (1) , Fanny Koëth (1) , Hugo Marnas (1) , Lydia Djenoune (1) , François-Xavier Lejeune (1) , Hervé Tostivint (2) , Claire Wyart (1)

1 ICM - Institut du Cerveau = Paris Brain Institute
2 PhyMA - Physiologie moléculaire et adaptation

Pharmacological experiments indicate that neuropeptides can effectively tune neuronal activity and modulate locomotor output patterns. However, their functions in shaping innate locomotion often remain elusive. For example, somatostatin has been previously shown to induce locomotion when injected in the brain ventricles but to inhibit fictive locomotion when bath-applied in the spinal cord in vitro. Here, we investigated the role of somatostatin in innate locomotion through a genetic approach by knocking out somatostatin 1.1 (sst1.1) in zebrafish. We automated and carefully analyzed the kinematics of locomotion over a hundred of thousand bouts from hundreds of mutant and control sibling larvae. We found that the deletion of sst1.1 did not impact acousto-vestibular escape responses but led to abnormal exploration. sst1.1 mutant larvae swam over larger distance, at higher speed and performed larger tail bends, indicating that Somatostatin 1.1 inhibits spontaneous locomotion. Altogether our study demonstrates that Somatostatin 1.1 innately contributes to slowing down spontaneous locomotion.

Domains: Life Sciences [q-bio]; Neurons and Cognition [q-bio.NC]; Neurobiology

Feng B Quan, Laura Desban, Olivier Mirat, Maxime Kermarquer, Julian Roussel, et al.. Somatostatin 1.1 contributes to the innate exploration of zebrafish larva. Scientific Reports, 2020, 10, pp.15235. ⟨10.1038/s41598-020-72039-x⟩. ⟨mnhn-02943951⟩