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Two high-quality de novo genomes from single ethanol-preserved specimens of tiny metazoans (Collembola)

Abstract : Abstract Background Genome sequencing of all known eukaryotes on Earth promises unprecedented advances in biological sciences and in biodiversity-related applied fields such as environmental management and natural product research. Advances in long-read DNA sequencing make it feasible to generate high-quality genomes for many non–genetic model species. However, long-read sequencing today relies on sizable quantities of high-quality, high molecular weight DNA, which is mostly obtained from fresh tissues. This is a challenge for biodiversity genomics of most metazoan species, which are tiny and need to be preserved immediately after collection. Here we present de novo genomes of 2 species of submillimeter Collembola. For each, we prepared the sequencing library from high molecular weight DNA extracted from a single specimen and using a novel ultra-low input protocol from Pacific Biosciences. This protocol requires a DNA input of only 5 ng, permitted by a whole-genome amplification step. Results The 2 assembled genomes have N50 values >5.5 and 8.5 Mb, respectively, and both contain ∼96% of BUSCO genes. Thus, they are highly contiguous and complete. The genomes are supported by an integrative taxonomy approach including placement in a genome-based phylogeny of Collembola and designation of a neotype for 1 of the species. Higher heterozygosity values are recorded in the more mobile species. Both species are devoid of the biosynthetic pathway for β-lactam antibiotics known in several Collembola, confirming the tight correlation of antibiotic synthesis with the species way of life. Conclusions It is now possible to generate high-quality genomes from single specimens of minute, field-preserved metazoans, exceeding the minimum contig N50 (1 Mb) required by the Earth BioGenome Project.
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Contributor : Cyrille d'Haese Connect in order to contact the contributor
Submitted on : Tuesday, October 5, 2021 - 11:18:08 AM
Last modification on : Saturday, November 27, 2021 - 3:49:30 AM
Long-term archiving on: : Thursday, January 6, 2022 - 6:29:57 PM


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Clément Schneider, Christian Woehle, Carola Greve, Cyrille d'Haese, Magnus Wolf, et al.. Two high-quality de novo genomes from single ethanol-preserved specimens of tiny metazoans (Collembola). GigaScience, BioMed Central, 2021, 10 (5), ⟨10.1093/gigascience/giab035⟩. ⟨mnhn-03365231⟩



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