Gut microbiota and holobiont metabolome composition of the Medaka fish (Oryzias latipes) are affected by a short exposure to the cyanobacterium Microcystis aeruginosa

Pierre Foucault (1) , Alison Gallet (1) , Charlotte Duval (1) , Benjamin Marie (1) , Sébastien Duperron (1)

1 MCAM - Molécules de Communication et Adaptation des Micro-organismes

Blooms of toxic cyanobacteria are a common stress encountered by aquatic fauna. Evidence indicates that long-lasting blooms affect fauna-associated microbiota. Because of their multiple roles, host-associated microbes are nowadays considered relevant to ecotoxicology, yet the respective timing of microbiota versus functional changes in holobionts response needs to be clarified. The response of gut microbiota and holobiont's metabolome to exposure to a dense culture of Microcystis aeruginosa was investigated as a microcosm-simulated bloom in the model fish species Oryzias latipes (medaka). Both gut microbiota and gut metabolome displayed significant composition changes after only 2 days of exposure. A dominant symbiont, member of the Firmicutes, plummeted whereas various genera of Proteobacteria and Actinobacteriota increased in relative abundance. Changes in microbiota composition occurred earlier and faster compared to metabolome composition. Liver and muscle metabolome were much less affected than guts, supporting that the gut and associated microbiota are in the front row upon exposure. This study highlights that even short cyanobacterial blooms, that are increasingly frequent, trigger changes in microbiota composition and holobiont metabolome. It emphasizes the relevance of multi-omics approaches to explore organism's response to an ecotoxicological stress.

Mots clés: Microbiota; Ecotoxicology; Multi-omics; Time-series; Cyanobacteria; Holobiont

Domaines: Ecotoxicologie; Biodiversité et Ecologie; Bactériologie

Pierre Foucault, Alison Gallet, Charlotte Duval, Benjamin Marie, Sébastien Duperron. Gut microbiota and holobiont metabolome composition of the Medaka fish (Oryzias latipes) are affected by a short exposure to the cyanobacterium Microcystis aeruginosa. Aquatic Toxicology, 2022, 253, pp.106329. ⟨10.1016/j.aquatox.2022.106329⟩. ⟨hal-04314150⟩