Encapsulation process of muscle stem cells in alginate matrices to enhance their in vivo post-transplant properties
Résumé
The identification of a type of stem cell derived from human adult muscle (hMuStem) and the demonstration of its regenerative potential have led to new cell therapy proposals for muscle diseases1,2. However, the ability of these cells to survive and integrate into the host tissue is a limiting factor in terms of their overall therapeutic impact. The emergence of tissue biomimetic approaches based on encapsulating cells in biocompatible and biodegradable biomaterials offers new opportunities to overcome the limitations of cell therapy in terms of viability and potentiation of effects. Initial in vitro results have enabled us to validate the possibility of encapsulating hMuStem cells in alginate matrices without altering the biological properties associated with their commitment in the myogenic program3. In addition, these results have made it possible to obtain millimeter-sized hydrogels using molding methods whose mechanical, structural and diffusion properties have been characterized as a function of the gelation methods used. However, the large size of these hydrogels limits their therapeutic applications to subcutaneous implants. For this reason, transposition of the encapsulation method to microfluidic approaches seems essential to achieve micrometric matrices more suitable for cell transplantation treatment applications4. So, to improve the efficiency of hMuStem cell delivery, we have built an on-chip microfluidic circuit compatible with their efficient encapsulation in micrometer-scale alginate matrices. In parallel, we assessed the impact of this encapsulation process on the morphology and viability of hMuStem cells
Domaines
Sciences du Vivant [q-bio]
Fichier principal
20240416 -poster Marquis Melanie format poster.pdf (1.46 Mo)
Télécharger le fichier